1,2‐Diacylglycerol accumulation in human neutrophils does not correlate with respiratory burst activation

FEBS Letters

Koenderman, Leo¹  Tool, Anton¹  Roos, Dirk¹  Verhoeven, Arthur J.¹ 

[1] Central Laboratory of the Netherlands Red Cross Blood Transfusion Service and Laboratory for Experimental and Clinical Immunology, University of Amsterdam, Amsterdam, The Netherlands

关键词: Neutrophil;    Respiratory burst;    Diacylglycerol;    1;    2-;    Serum-treated zymosan;   

DOI  :  10.1016/0014-5793(89)80170-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Measurements of the level of 1,2-diacylglycerol (1,2-DG during activation of the respiratory burst of human neutrophils by formyl-methionyl-leucyl-phenylalanine (fMLP in the presence of platelet-activating factor (PAF or by opsonized particles show that a correlation between accumulation of 1,2-DG and O₂ consumption does not exist. Inhibition of protein kinase C activity with staurosporine before addition of opsonized particles demonstrates that the first phase of the respiratory burst is not inhibited, whereas the second phase, which is accompanied by a rise in the content of 1,2-DG, is strongly inhibited. This study indicates that accumulation of 1,2-DG cannot be the sole signal for the initiation of the respiratory burst in human neutrophils.

【 授权许可】

Unknown