A new heparin‐inhibited and polyamine‐activated protein kinase from bovine kidney

FEBS Letters

Singh, Toolsee J.¹ 

[1] Department of Biology, University of Waterloo, Waterloo, Ontario N2L 3G1, Canada

关键词: Casein kinase-1;    Heparin inhibition;    Spermine activation;    Casein kinase-2;    Protein kinase;    Casein kinase-3;   

DOI  :  10.1016/0014-5793(89)80147-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Two casein kinases, casein kinase-1 (CK-1) and casein kinase-2 (CK-2), have been characterized from many sources. In this study we describe the properties of a third casein kinase, designated casein kinase-3 (CK-3). CK-3 (M _r 32 000) is readily separated from CK-2 by gel filtration and from CK-1 by hydroxyapatite chromatography. CK-3 phosphorylates several proteins, including phosphorylase kinase. Phosphorylation of phosphorylase kinase by CK-3 results in a 10-fold enzyme activation. CK-3 is activated by spermine and inhibited by heparin, ADP, and divalent metal ions (Mn²⁺, Zn²⁺). Heparin inhibition of the kinase is reversed by spermine. The physical and regulatory properties of CK-3 are very similar to CK-1, suggesting that these kinases may be closely related.

【 授权许可】

Unknown