The ATP,Mg‐dependent protein phosphatase: Regulation by casein kinase‐1

FEBS Letters

Goris, Jozef¹  Merlevede, Wilfried¹  Vandenheede, Jackie R.¹  Pinna, Lorenzo A.²  Agostinis, Patrizia¹  Meggio, Flavio² 

[1] Afdeling Biochemie, Faculteit Geneeskunde, Katholieke Universiteit te Leuven, Leuven 3000, Belgium;Istituto di Chimica Biologica, Università di Padova, Padova 35131, Italy

关键词: ATP;    Mg-dependent phosphatase;    Casein kinase-1;    Casein kinase-2;    Modulator subunit;   

DOI  :  10.1016/0014-5793(87)80489-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The free modulator subunit of the ATP,Mg-dependent phosphatase is phosphorylated up to 1 mol per mol by casein kinase-1, up to 1.85 mol per mol after dephosphorylation by the PCS_H1 phosphatase, but 10-fold less when purified in the presence of NaF, suggesting an in vivo phosphorylation of the casein kinase-1 sites. Peptide mapping of ³²P-modulator labeled by casein kinase-1 or -2 shows a different phosphorylation pattern. Phosphorylation of the inactive phosphatase by casein kinase-1 prevents the subsequent kinase F_A-mediated activation, while it does not impair the activated phosphatase.

【 授权许可】

Unknown