Developmental regulation of five subunit specific mRNAs encoding acetylcholine receptor subtypes in rat muscle

FEBS Letters

Sakmann, Bert¹  Stein, Elke¹  Witzemann, Veit¹  Criado, Manuel¹  Barg, Brigitte¹ 

[1] Max-Planck-Institut für biophysikalische Chemie, Postfach 2841, D-34 Göttingen, FRG

关键词: Acetylcholine receptor;    Development;    Channel subtype;    RNA blot hybridization;   

DOI  :  10.1016/0014-5793(89)80514-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The muscular content of the mRNAs encoding the five subunits of the nicotinic acetylcholine receptor was measured during postnatal development in the rat. Subunit specific mRNAs show differential regulation. The levels of the α-, γ- and δ-subunit specific mRNAs decrease steadily after birth, while the β and ε-subunit mRNAs increase transiently and then decrease. The adult pattern of subunit specific mRNA levels is reached at 4–6 weeks postnatally. The content of γ- and ε-subunit mRNA changes in a reciprocal fashion during the first 2 postnatal weeks, supporting the view that differential regulation of γ- and ε-subunit mRNA during development is one mechanism mediating the appearance of the adult, ε-subunit containing, subtype of end-plate channel. Denervation of neonatal muscle increases the levels of all subunit-specific mRNAs during further development. It prevents the postnatal decrease in γ-subunit mRNA and enhances the initial increase in ε-subunit mRNA. This makes it appear that the ε-subunit gene is less sensitive to regulation by the nerve in the postnatal period than the γ-subunit gene.

【 授权许可】

Unknown