XANES spectroscopy sensitivity to small electronic changes Case of carp azidomethemoglobin

FEBS Letters

Cortes, Robert¹  Pin, Serge²  Alpert, Bernard³  Le Tilly, Véronique² 

[1] Laboratoire de Physique des Liquides et Electrochimie, CNRS LP 15, associé à l'Université Pierre et Marie Curie, 75231 Paris Cedex 05 France;Laboratoire de Biologie Physico-Chimique, Université Paris VII, 2 place Jussieu, 75251 Paris Cedex 05 France;LURE (CNRS LP 8, CEA, MEN), Université Paris Sud, Bâtiment 209, 91405 Orsay Cedex, France

关键词: Iron K-edge spectrum;    Spin equilibrium;    Hemoglobin;   

DOI  :  10.1016/0014-5793(89)80510-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Spin states equilibrium of hemoglobin-iron varies with external conditions: pH, allosteric effectors, temperature. The small electronic reorganization of the iron caused by the spin state changes has been detected by X-ray absorption near edge structure (XANES) spectroscopy at room temperature. The iron K-edge region which is sensitive to spin state is located in 7110–7130 eV. Here are presented the 100% high spin and 100% low spin XANES spectra of carp azido ferric hemoglobin.

【 授权许可】

Unknown