Spin equilibrium relaxation kinetics of cytochrome P450 LM2

FEBS Letters

Ruckpaul, K.¹  Blanck, J.¹  Ziegler, M.¹ 

[1] Department of Biocatalysis, Central Institute of Molecular Biology, GDR Academy of Sciences, 1115 Berlin-Buch, GDR

关键词: Cytochrome P450;    Laser;    Temperature-jump;    Spin equilibrium;    Relaxation kinetics;    Reaction mechanism;   

DOI  :  10.1016/0014-5793(82)81338-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The relaxation kinetics of the temperature-dependent spin equilibrium of cytochrome P450 LM2 has been resolved by means of a laser temperature-jump device. The Nd laser system in the Q-switched mode delivers 3 J/25 ns pulses which were Raman shifted to 1.89 μm by use of a high pressure H₂-gas cell. The LM2 equilibrium relaxation proceeds in the ns-range, the rate constants of the on- and off-reactions amount to about 10⁶ s⁻¹ in correspondence with respective processes of other hemoproteins and protein-free model compounds. Benzphetamine increases the on-rate constant (low-spin → high-spin) about 5 fold, the off-process is nearly unchanged.

【 授权许可】

Unknown