Evidence for a type II topoisomerase in wheat chloroplasts

FEBS Letters

Leech, R.M.¹  Marrison, J.¹  Pyke, K.A.¹ 

[1] Department of Biology, University of York, Heslington, York Y01 5DD, England

关键词: Topoisomerase;    Chloroplast DNA;    Chloroplast development;    Western blotting;    (Mesophyll cell);   

DOI  :  10.1016/0014-5793(89)80490-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Western blotting of total cell extracts from the young developing wheat leaf with an antibody to yeast topoisomerase II locates two proteins of molecular mass 96–101 kDa which also appear on blots of chloroplast proteins. The maximal levels of both proteins are present in chloroplasts in which chloroplast DNA replication is occurring prior to chloroplast division.

【 授权许可】

Unknown