Somatostatin activates glibenclamide‐sensitive and ATP‐regulated K+ channels in insulinoma cells via a G‐protein

FEBS Letters

Lazdunski, Michel¹  Schmid-Antomarchi, Heidy¹  Fosset, Michel¹  de Weille, Jan R.¹ 

[1] Centre de Biochimie, Centre National de la Recherche Scientifique, Parc Valrose, 06034 Nice Cedex, France

关键词: Somatostatin;    Glibenclamide;    86Rb+ efflux;    G-protein;    (RINm5F cell);   

DOI  :  10.1016/0014-5793(88)80992-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Somatostatin, an hyperglycemia-inducing hormone, was studied in rat insulinoma (RINm5F) cells using ⁸⁶Rb⁺ efflux techniques. ⁸⁶Rb⁺ efflux is stimulated by somatostatin in a dose-dependent manner. The half-maximum value of activation is 0.7 nM. Somatostatin-induced ⁸⁶Rb⁺ efflux is abolished by the hypoglycemia-inducing sulfonylurea, glibenclamide, a known blocker of ATP-regulated K⁺ channels. Somatostatin activation is prevented by pretreatment of insulinoma cells with pertussis toxin. ⁸⁶Rb⁺ efflux studies show that somatostatin activates an ATP-dependent K⁺ channel.

【 授权许可】

Unknown