Effects of glucose on insulin release and 86Rb permeability in cultured neonatal and adult rat islets

FEBS Letters

Atwater, Illani¹  Boschero, A.Carlos¹  Tombaccini, Donatella¹ 

[1] Laboratory of Cell Biology, and Genetics, NIDDK, Bldg 8/Rm 403, National Institutes of Health, Bethesda, MD 20892, USA

关键词: Insulin release;    86Rb+ efflux;    K+ permeability;    Glucose;    (B-cell;    Islets of Langerhans;    Rat);   

DOI  :  10.1016/0014-5793(88)80059-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Glucose-induced insulin release and modifications in ⁸⁶Rb outflow were studied in cultured neonatal and adult rat islets. The dose-response curve for neonatal islets was steeper than for adult islets and the maximal response was clearly shifted towards lower glucose concentrations. In neonatal islets, glucose-induced insulin release was inhibited by the Ca²⁺-channel blocker, nifedipine. In the absence of glucose, the ⁸⁶Rb outflow from neonatal islets was lower than from adult islets. Also, the glucose-induced reduction in ⁸⁶Rb outflow was less pronounced in neonatal islets. Altered K⁺ permeability in the B-cell membrane could explain the change in glucose sensitivity of neonatal islets.

【 授权许可】

Unknown