Oxidation of rat insulin II, but not I, leads to anomalous elution profiles upon HPLC analysis of insulin‐related peptides

FEBS Letters

Weir, Gordon¹  Hendrick, Grant¹  Skvorak, Anne³  Villa-Komaroff, Lydia²  Halban, Philippe³  Gross, David¹ 

[1] Joslin Diabetes Center and Brigham and Women's Hospital, Harvard Medical School, Boston, USA;Department of Neuroscience, Children's Hospital, Harvard Medical School, Boston, USA;Laboratoires de Recherche Louis Jeantet, Geneva University School of Medicine, Geneva, Switzerland

关键词: Insulin biosynthesis;    HPLC;    Methionine sulfoxide;    (Rat);   

DOI  :  10.1016/0014-5793(88)81062-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Rat insulin II, unlike rat insulin I and other non-rodent insulins, contains a unique methionine residue at position B29. Reversed-phase HPLC allows for separation of the two rat insulins, with insulin I typically eluting faster than insulin II. An anomalous peak of insulin immunoreactive material was found eluting even faster than insulin I following acid extraction of rat insulin-producing cells. This early peak co-eluted with [Met-O ^B29]insulin II suggesting that during cell extraction and subsequent purification steps, rat insulin II is subject to selective oxidation at Met^B29. Such oxidation of rat proinsulin II affords improved separation from rat proinsulin I compared to the native form.

【 授权许可】

Unknown