Calcium mobilization by angiotensin II and neurotransmitter receptors expressed in Xenopus laevis oocytes

FEBS Letters

Sandberg, Kathryn¹  Catt, Kevin J.¹  Trinh, Dorothy P.¹  Markwick, Alison J.¹ 

[1] Endocrinology and Reproduction Research Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892 USA

关键词: Ca2+ mobilization;    Oocyte;    Aequorin;    Angiotensin II receptor;    (Xenopus laevis);   

DOI  :  10.1016/0014-5793(88)81055-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Specific receptors for angiotensin II (AII) were expressed in albino Xenopus laevis oocytes co-injected with poly(A)⁺ mRNA isolated from rat adrenal cortex and the calcium-specific photoprotein, aequorin. In such oocytes, AII elicited rapid, dose-dependent rises in cytosolic free calcium with light emission responses up to 100-fold above basal levels. Ligand-induced light emission was also observed in oocytes injected with rat brain mRNA and stimulated with acetylcholine and glutamate. These findings demonstrate that mammalian AII receptors expressed in Xenopus oocytes are functionally linked to intracellular Ca²⁺ mobilization, and indicate the potential value of aequorin-injected oocytes for rapid and specific screening of mRNAs transcribed from expression libraries containing cloned receptor cDNAs.

【 授权许可】

Unknown