Flagellin parts acquiring a regular structure during polymerization are disposed on the molecule ends

FEBS Letters

Kostyukova, A.S.¹  Fedorov, O.V.¹  Pyatibratov, M.G.¹  Filimonov, V.V.¹ 

[1] Institute of Protein Research, Academy of Sciences of the USSR, 142292 Pushchino, Moscow Region, USSR

关键词: Flagellin;    Limited proteolysis;    Microcalorimetry;    (Escherichia coli);    SDS;    sodium dodecyl sulfate;    PAGE;    polyacrylamide gel electrophoresis;   

DOI  :  10.1016/0014-5793(88)81047-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Flagellins of two Escherichia coli strains have been investigated by limited proteolysis and scanning microcalorimetry. It has been shown that a monomer flagellin consists of two parts: a central one cooperatively melting, rather resistant to proteases, and the other without a stable tertiary structure and thus easily degrading terminals. Just these terminals acquire a regular structure during polymerization. Core fragments of the two strains have been isolated and characterized.

【 授权许可】

Unknown