The influence of culture medium composition on drug metabolising enzyme activities of the human liver derived Hep G2 cell line

FEBS Letters

Melvin, W.T.³  Burke, M.D.¹  Grant, M.H.²  Doostdar, H.²  Duthie, S.J.² 

[1] Clinical Pharmacology Unit, Department of Pharmacology, University of Aberdeen, Polwarth Building, Foresterhill, Aberdeen AB9 2ZD, Scotland;Clinical Pharmacology Unit, Departments of Medicine & Therapeutics, University of Aberdeen, Polwarth Building, Foresterhill, Aberdeen AB9 2ZD, Scotland;Clinical Pharmacology Unit, Department of Biochemistry, University of Aberdeen, Polwarth Building, Foresterhill, Aberdeen AB9 2ZD, Scotland

关键词: Cytochrome P-450;    UDP-glucuronyltransferase;    Glutathione;    Glutathione-S-transferase;    (Human Hep G2 hepatoma cell;    Cell culture);    EROD;    ethoxyresorufin O-dealkylation;    MROD;    methoxyresorufin O-dealkylation;    PROD;    pentoxyresorufin O-dealkylation;    BROD;    benzyloxyresorufin O-dealkylation;    MFO;    mixed function oxidase;    GT;    UDP-glucuronyltransferase;    GST;    glutathione-S-transferase;    GSH;    reduced glutathione;    CDNB;    1-chloro-2;    4-dinitrobenzene;   

DOI  :  10.1016/0014-5793(88)81021-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

When grown in the standard Dulbecco's medium the human liver derived Hep G2 hepatoma cell line shows only 10–20% of the cytochrome P-450-dependent mixed function oxidase (MFO) activity of freshly isolated human adult hepatocytes. However, the MFO activities and, to a lesser extent, the activities of UDP-glucuronyltransferase and glutathione-S-transferase can be increased by altering the composition of the growth medium. Modified Earle's medium was more effective in this respect than Williams' E medium and increased the O-dealkylations of ethoxyresorufin, benzyloxyresorufin and pentoxyresorufin 50-, 30- and 10-fold, respectively.

【 授权许可】

Unknown