Different sensitivities to agonist of muscarinic acetylcholine receptor subtypes

FEBS Letters

Akiba, Isamu¹  Mishina, Masayoshi¹  Nakai, Junichi¹  Bujo, Hideaki¹  Maeda, Akito¹  Numa, Shosaku¹  Fukuda, Kazuhiko¹  Kubo, Tai¹ 

[1] Departments of Medical Chemistry and Molecular Genetics, Kyoto University, Faculty of Medicine, Kyoto 606, Japan

关键词: Muscarinic acetylcholine receptor subtype;    Ca2+-dependent Cl− current;    Na+ current;    K+ current;    Phosphoinositide hydrolysis;    Sensitivity to agonist;    Agonist-binding affinity;    mAChR;    muscarinic acetylcholine receptor;    ACh;    acetylcholine;    NMS;    N-methylscopolamine;    QNB;    quinuclidinyl benzilate;   

DOI  :  10.1016/0014-5793(88)80346-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Muscarinic acetylcholine receptor (mAChR) III expressed in Xenopus oocytes, like mAChR I, mediates activation of a Ca²⁺-dependent Cl⁻ current, whereas mAChR IV, like mAChR II, principally induces activation of Na⁺ and K⁺ currents in a Ca²⁺-independent manner. mAChR III has a sensitivity to agonist of about one order of magnitude higher than that of mAChR I in mediating the Ca²⁺-dependent current response in Xenopus oocytes and in stimulating phosphoinositide hydrolysis in NG108-15 neuroblastoma-glioma hybrid cells. The agonist-binding affinity of mAChR III is also about one order of magnitude higher than that of mAChR I.

【 授权许可】

Unknown