An enzyme which specifically splits a covalent bond between picornaviral RNA and VPg

FEBS Letters

Drygin, Yuri¹  Bogdanov, Alexei¹  Shabanov, Alexei¹ 

[1] A.N. Belozersky Laboratory of Molecular Biology and Bioorganic Chemistry, Moscow State University, Moscow 119899, USSR

关键词: RNA-protein complex;    Picornavirus;    Comovirus;    Heteropolymeric covalent bond cleavage;   

DOI  :  10.1016/0014-5793(88)80948-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

An enzyme which specifically splits a covalent bond between VPg and picornaviral RNAs (tentatively designated Y-pUpN PDE) has been partially purified from mouse ascites Krebs II cells. Using substrates labelled in vitro with¹²⁵I-Bolton-Hunter reagent and a new assay based on Kieselgel thin-layer chromatography, several biochemical characteristics of the enzyme have been determined, depending on the pH and on Mg²⁺, K⁺, spermidine and PEG concentrations, etc. We found that the enzyme does not ‘unlink’ VPg from comoviral RNA. We suggest that Y-pUpN PDE represents a new class of enzymes.

【 授权许可】

Unknown