FEBS Letters | |
An enzyme which specifically splits a covalent bond between picornaviral RNA and VPg | |
Drygin, Yuri1  Bogdanov, Alexei1  Shabanov, Alexei1  | |
[1] A.N. Belozersky Laboratory of Molecular Biology and Bioorganic Chemistry, Moscow State University, Moscow 119899, USSR | |
关键词: RNA-protein complex; Picornavirus; Comovirus; Heteropolymeric covalent bond cleavage; | |
DOI : 10.1016/0014-5793(88)80948-7 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
An enzyme which specifically splits a covalent bond between VPg and picornaviral RNAs (tentatively designated Y-pUpN PDE) has been partially purified from mouse ascites Krebs II cells. Using substrates labelled in vitro with125I-Bolton-Hunter reagent and a new assay based on Kieselgel thin-layer chromatography, several biochemical characteristics of the enzyme have been determined, depending on the pH and on Mg2+, K+, spermidine and PEG concentrations, etc. We found that the enzyme does not ‘unlink’ VPg from comoviral RNA. We suggest that Y-pUpN PDE represents a new class of enzymes.
【 授权许可】
Unknown
【 预 览 】
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RO201912020291263ZK.pdf | 549KB | download |