The first crystallization of a vanadium‐dependent peroxidase

FEBS Letters

Vilter, H.²  Müller-Fahrnow, A.¹  Hinrichs, W.¹  Saenger, W.¹ 

[1] Institut für Kristallographie, Freie Universität Berlin, Takustr. 6, D-1000 Berlin 33, Germany;Institut für Pharmazeutische Biologie der Universität Bonn, Nussallee 6, D-5300 Bonn 1, FRG

关键词: Peroxidase;    Vanadium(V);    Crystallization;    (Seaweed;    Ascophyllum nodosum);   

DOI  :  10.1016/0014-5793(88)80937-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Single crystals of a vanadium-containing peroxidase from the brown alga Ascophyllum nodosum have been grown by the vapour diffusion technique using polyethylene glycol 6000 along with sodium chloride as precipitant. The crystals belong to the monoclinic space group P2₁. X-ray diffraction extends to at least 2.4 Å. The cell dimensions a = 173.0, b = 164.9, c=68.5 Å, β=94.5° indicate that there are four molecules of 100 kDa per asymmetric unit, suggesting that the native enzyme might occur as a tetramer.

【 授权许可】

Unknown