A time‐course study on superoxide generation and protein kinase C activation in human neutrophils

FEBS Letters

Christiansen, Niels Obel¹ 

[1] Department of Medicine and Infectious Diseases, Marselisborg Hospital, University of Aarhus, P.P. Ørumsgade 11, DK-8000 Aarhus C, Denmark

关键词: Superoxide;    Protein kinase C;    Phorbol ester;    Leukocyte;    Membrane association;    PMA;    phorbol 12-myristate 13-acetate;    FMLP;    N-formyl-methionyl-leucyl-phenylalanine;   

DOI  :  10.1016/0014-5793(88)80915-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The time course of superoxide generation and membrane association of protein kinase C was studied in human neutrophils stimulated by PMA, FMLP, ionomycin and A23187. The initiation of superoxide generation in PMA; ionomycin- and A23187-stimulated neutrophils was characterized by a lag period of at least 30 s in contrast to a lag period of 10–15 s in FMLP-stimulated cells. The time course of membrane association of protein kinase C in PMA-stimulated neutrophils was highly dependent upon the PMA concentration used for stimulation. However, membrane association of protein kinase C preceded superoxide generation in cells stimulated by 10–300 ng/ml PMA. FMLP, ionomycin and A23187 induced membrane association of protein kinase C in a few seconds and always before superoxide generation. It is concluded that membrane association of protein kinase C in PMA-, FMLP-, ionomycin- and A23187-stimulated neutrophils precedes superoxide generation, and thereby may be part of the mechanism initiating NADPH-oxidase activity. A simple correlation between the two parameters could not be proven, indicating that also other activation mechanisms are decisive in the activation of NADPH-oxidase.

【 授权许可】

Unknown