Catalase negative Staphylococcus aureus retain virulence in mouse model of chronic granulomatous disease

FEBS Letters

Roes, Jürgen¹  Messina, Carlo G.M²  Segal, Anthony W²  Reeves, Emer P² 

[1] The Windeyer Institute of Medical Sciences, 46 Cleveland Street, London W1P 6DB, UK;Centre for Molecular Medicine, The Rayne Institute, University College London, 5 University Street, London WC1E 6JJ, UK

关键词: Superoxide;    Chronic granulomatous disease;    Staphylococcus;    Catalase;    Infection;    Leukocyte;    CGD;    chronic granulomatous disease;    cat;    catalase;    MPO;    myeloperoxidase;    CFU;    colony forming units;   

DOI  :  10.1016/S0014-5793(02)02658-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Myeloperoxidase-mediated chlorination is thought to be a necessary microbicidal mechanism. The H₂O₂ required for this process is generated by the NADPH oxidase. Staphylococcus aureus can also produce H₂O₂, which is not broken down by catalase negative organisms. It has been thought that this bacterial H₂O₂ can substitute for cellular H₂O₂ in the halogenation reaction in chronic granulomatous disease (CGD) where neutrophils are lacking the NADPH oxidase. We have readdressed this issue in a mouse model of CGD using clinical isolates of catalase positive and negative strains of S. aureus. The results showed these organisms to be equally virulent and that the H₂O₂ they produced is insufficient to cause significant iodination, a marker for chlorination, thereby contradicting the accepted views on this subject.

【 授权许可】

Unknown