Adenosine 3′,5′‐cyclic monophosphate (cAMP) inhibits phorbol ester‐induced growth of an IL‐2‐dependent T cell line

FEBS Letters

Takeshita, Toshikazu¹  Goto, Yuso¹  Sugamura, Kazuo¹ 

[1] Department of Bacteriology, Tohoku University School of Medicine, Sendai 980, Japan

关键词: cyclic AMP;    Protein kinase C;    Interleukin-2;    cAMP;    adenosine 3′;    5′-cyclic monophosphate;    TPA;    12-O-tetradecanoylphorbol 13-acetate;    PDBu;    phorbol 12;    13-dibutyrate;    PKC;    protein kinase C;    IBMX;    1-methyl-3-isobutylxanthine;    db-cAMP;    dibutyryl-cAMP;    8b-cAMP;    8-bromo-cAMP;    PI;    phosphatidylinositol;    IL-2;    interleukin 2;    PKA;    protein kinase A;   

DOI  :  10.1016/0014-5793(88)80909-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We previously established a human T cell line, TPA-Mat, which can proliferate in response to not only interleukin-2 (IL-2), but also phorbol esters such as 12-O-tetradecanoylphorbol-13-acetate (TPA) and phorbol-12,13-dibutyrate (PDBu). The present study demonstrated that the PDBu-dependent growth of TPA-Mat cells was inhibited up to 90% by adenosine 3′,5′-cyclic monophosphate (cAMP) raising agents such as forskolin, cholera toxin and 1-methyl-3-isobutylxanthine, and cAMP analogues, whereas the IL-2-stimulated TPA-Mat growth was slightly inhibited. These findings suggest that the signal transduction pathway of PDBu-induced growth, which should involve activation of protein kinase C, is sensitive to cAMP, and that it cannot be exactly identical to the signal transduction pathway of Il-2-induced growth in TPA-Mat cells.

【 授权许可】

Unknown