Thermodynamic parameters of the binding of the tight‐binding I12X86 lac repressor to operator and non‐operator DNA

FEBS Letters

Grebert, Philippe¹  Maurizot, Jean Claude¹ 

[1] Centre de Biophysique Moleculaire, 1A, Avenue de la Recherche Scientifique, 45071 Orleans Cédex, France

关键词: Lac repressor;    Lac operator;    DNA binding;    Thermodynamic parameter;   

DOI  :  10.1016/0014-5793(88)80554-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The thermodynamic parameters ΔH and ΔS corresponding to the binding of the tight-binding double mutant lac repressor I12X86 with operator and non-operator DNA fragments were determined using the nitrocellulose filter binding assay. In both cases the binding processes are entropically driven and accompanied by an unfavorable enthalpy variation. The differences between these parameters and those previously reported for the wild type lac repressor show that the strategy adopted by the mutant to interact with DNA is highly different from that of the wild type repressor and suggest more hydrophobic contacts between the mutant and DNA.

【 授权许可】

Unknown