期刊论文详细信息
Virology Journal
Nuclear domain 10-associated proteins recognize and segregate intranuclear DNA/protein complexes to negate gene expression
Qiyi Tang1  Bruno R Rojas1  Yisel A Rivera-Molina1 
[1] Department of Microbiology/RCMI Program, Ponce School of Medicine and Health Sciences, Ponce, 00716, Puerto Rico
关键词: Nuclear bodies;    Transcript location;    Daxx;    PML;    Lac repressor;    Lac operator;    Amplicon;    Protein-DNA complexes;    Nuclear domain 10 (ND10);   
Others  :  1153646
DOI  :  10.1186/1743-422X-9-222
 received in 2012-07-10, accepted in 2012-09-27,  发布年份 2012
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【 摘 要 】

Background

DNA viruses, such as herpes simplex virus type 1 (HSV-1), Simian virus 40 (SV40), and Cytomegaloviruses (CMV), start their replicative processes and transcription at specific nuclear domains known as ND10 (nuclear domain 10, also called PML bodies). It has been previously determined that for HSV-1 and SV40, a short DNA sequence and its binding protein are required and sufficient for cell localization of viral DNA replication and gene transcription.

Results

Our recent observations provide evidence that a foreign (not endogenous) DNA/protein complex in the nucleus recruits ND10 proteins. First, the complexes formed from the bacterial lac operator DNA and its binding protein (lac repressor), or from HPV11 (human papillomavirus 11) origin DNA and its binding protein (E2), co-localized with different ND10 proteins. Second, the HSV-1 amplicon without inserted lac operator DNA repeats distributed in the nucleus randomly, whereas the amplicon with lac operator DNA repeats associated with ND10, suggesting that DNA-binding proteins are required to localize at ND10. The cellular intrinsic DNA/protein complex (as detected for U2 DNA) showed no association with ND10. Furthermore, our examination of PML−/−, Daxx−/−, and Sp100-negative cells led to our discovering that DNA/protein complexes recruit ND10 protein independently. Using the GFP-LacI/Operator system, we were able to direct the transfected DNA to ND10 and found that gene expression was significantly repressed when the transfected DNA was directed to ND10.

Conclusion

Taken together, the results suggest that cells recognize DNA/protein complexes through a mechanism that involves interaction with the ND10-associated proteins.

【 授权许可】

   
2012 Rivera-Molina et al.; licensee BioMed Central Ltd.

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