The herbicidally active experimental compound Hoe 704 is a potent inhibitor of the enzyme acetolactate reductoisomerase

FEBS Letters

Köcher, Helmut¹  Spönemann, Petra¹  Schulz, Arno¹  Wengenmayer, Friedrich¹ 

[1] Hoechst AG, 6230 Frankfurt/Main 80, FRG

关键词: Acetolactate reductoisomerase;    Enzyme inhibition;    Herbicide;    Hoe 704;    AHB;    2-aceto-2-hydroxybutyrate;    AL;    2-acetolactate;    PMSF;    phenylmethanesulfonyl fluoride;   

DOI  :  10.1016/0014-5793(88)80515-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Growth inhibition of plants and bacteria by the experimental herbicide Hoe 704 (2-methylphosphinoyl-2-hydroxyacetic acid) was alleviated by the addition of the branched-chain amino acids to growth media. Hoe 704 caused a massive accumulation of acetoin and acetolactate, indicating its direct interference with the branched-chain amino acid biosynthetic pathway. The second enzyme of this pathway, acetolactate reductoisomerase (EC 1.1.1.86), was found to be subject to strong inhibition by Hoe 704. The inhibition was time-dependent and competitive with the enzyme's substrate, acetolactate. This report establishes acetolactate reductoisomerase as a new target for a herbicidal compound.

【 授权许可】

Unknown