Isolation and analysis of the mouse opsin gene

FEBS Letters

Baehr, W.²  Bugra, K.¹  Falk, J.D.²  McGinnis, J.F.¹  Triantafyllos, J.T.¹ 

[1] Department of Anatomy and The Mental Retardation Research Center, UCLA School of Medicine, Los Angeles, CA 90024, USA;Department of Biological Sciences, Purdue University, West Lafayette, IN 47907, USA

关键词: Opsin;    Visual pigment;    cDNA cloning;    Gene cloning;    Peptide sequence;    (Mouse retina);   

DOI  :  10.1016/0014-5793(88)80490-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have identified three overlapping 5′-truncated mouse opsin cDNA clones by immunologically screening a λgt11 retina expression library. Using one of the cDNA clones as a probe, we isolated a 5 kb genomic fragment that encompassed the complete coding sequence for mouse opsin. The coding region for opsin was interrupted by four introns positioned precisely as those previously described for other mammalian opsins. In contrast to the single major opsin mRNA in the bovine and human retina, Northern analysis of mouse retina RNA demonstrated the presence of at least five distinct species of polyadenylated opsin mRNAs. Their sizes ranged from 1.7 kb to 5.1 kb.

【 授权许可】

Unknown