Mechanistic studies on the phosphorylation of photoexcited rhodopsin

FEBS Letters

Akhtar, M.¹  Sharma, Ram¹  Fowles, Charles¹ 

[1] Department of Biochemistry, University of Southampton, Bassett Crescent East, Southampton SO9 3TU, England

关键词: Rhodopsin;    Rod outer segment;    Rhodopsin kinase;    Synthetic peptide;    Peptide phosphorylation;    (Retina);   

DOI  :  10.1016/0014-5793(88)80224-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The mechanism of the photophosphorylation of rhodopsin was studied using several synthetic peptides corresponding to the sequence of the phosphorylation domain. It was found that the decapeptide (residues 339–348) was effectively phosphorylated by rhodopsin kinase only when incubation was performed in the presence of both rhodopsin and light. These results are interpreted to suggest that in the dark-adapted state rhodopsin kinase exists in an inactive conformation and that this is converted into a catalytically competent form only after interaction with metarhodopsin II (Rho*)

【 授权许可】

Unknown