Kinetic studies on the function of A1 in the photosystem I reaction center

FEBS Letters

Mathis, P¹  Sétif, P¹ 

[1] Service de Biophysique, Département de Biologie, CEN Saclay, 91191 Gif-sur-Yvette Cedex, France

关键词: Photosystem I;    Reaction center;    Flash absorption spectroscopy;    Electron transfer;    Kinetics;    PS I;    photosystem I;    DCIP;    dichlorophenolindophenol;   

DOI  :  10.1016/0014-5793(88)80173-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Flash absorption measurements with nanosecond time resolution were conducted in the near-ultraviolet with subchloroplast particles enriched in photosystem I, at 6°C. Around 370 nm, an absorption increase decays biphasically, with a fast phase, t = 15 ± 5 ns. Control experiments show that this rapid phase is due to the reoxidation of A₁ (presumably phylloquinone), which thus appears to function as an electron carrier in PS I at physiological temperature. The nanosecond kinetic phase is not altered if the iron-sulfur centers F_A and F_B are kept reduced.

【 授权许可】

Unknown