Two ionic forms of exoglucanase in yeast secretory mutants

FEBS Letters

Hernández, Luis M.¹  Larriba, Germán¹  Olivero, Isabel¹  Ramírez, Manuel¹  Basco, Ricardo D.¹ 

[1] Departamento de Microbiología, Facultad de Ciencias, Universidad de Extremadura, 06071 Badajoz, Spain

关键词: Exoglucanase;    Secretory pathway;    Ionic form;    (Yeast);    endo H;    endo-β-N-acetylglucosaminidase H;    PAGE;    polyacrylamide gel electrophoresis;    BSA;    bovine serum albumin;    TBS;    Tris-buffered saline;    ER;    endoplasmic reticulum;   

DOI  :  10.1016/0014-5793(88)80170-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Analysis of exoglucanase activity accumulated by sec mutants from Saccharomyces cerevisiae revealed the presence of two ionic forms of the major exoglucanase (exo II) secreted into the culture medium. From the accumulation pattern of representative sec mutants and the carbohydrate composition it appears that the less acidic form is converted into the more acidic one by addition of one phosphate to one of the oligosaccharide cores as the enzyme progresses through the secretory pathway. Exoglucanase I, the heavier isoenzyme, was not accumulated by the mutants. Accordingly, it should arise from exoglucanase II after the execution point of sec1 mutation.

【 授权许可】

Unknown