Accumulation of exoglucanase activity in yeast secretory mutants blocked at the endoplasmic reticulum level

FEBS Letters

Hernández, Luis M.¹  Larriba, Germán¹  Olivero, Isabel¹  Ramírez, Manuel¹ 

[1] Departamento de Microbiologia, Facultad de Ciencias, Universidad de Extremadura, 06071 Badajoz, Spain

关键词: Exoglucanase (Saccharomyces cerevisiae) Secretory mutant Endoplasmic reticulum;   

DOI  :  10.1016/0014-5793(86)80265-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Saccharomyces cerevisiae HMSF-176 (sec 18), a thermosensitive secretory mutant blocked at the endoplasmic reticulum (ER) level, drastically increased its osmotic sensitivity when grown at the restrictive temperature of 37°C in high glucose concentration. This fact led to the erroneous interpretation that glucanases were inactive when localized in the ER. The development of a suitable osmotic stabilizer now indicates that sec 18 accumulates exoglucanase activity. Another ER-blocked mutant behaved in a similar way. All the accumulated exoglucanase was found in a soluble form. By contrast, a significant portion of the accumulated invertase remained in a membrane-bound form.

【 授权许可】

Unknown