Primary structure of human insulin‐like growth factor‐binding protein/placental protein 12 and tissue‐specific expression of its mRNA

FEBS Letters

Koistinen, Riitta¹  Jänne, Olli A.²  Seppälä, Markku¹  Aalto-Setälä, Katriina³  Kontula, Kimmo¹  Julkunen, Mervi² 

[1] Department of Obstetrics and Gynecology, University of Helsinki, SF-00290 Helsinki Finland;The Population Council and The Rockefeller University, New York, NY 10021, USA;Recombinant DNA Laboratory, University of Helsinki, SF-00380 Helsinki, Finland

关键词: Insulin-like growth factor-binding protein;    Placental protein 12;    Amino acid sequence;    mRNA expression;   

DOI  :  10.1016/0014-5793(88)80041-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The low-molecular-mass insulin-like growth factor-binding protein (IGF-BP) and placental protein 12 (PP12) are identical proteins that are present in human serum, amniotic fluid, secretory endometrium and decidua. IGF-BP/PP12 is believed to act as an autocrine or paracrine regulator of cell growth. A cDNA clone encompassing the entire protein coding region of this protein was isolated from a human decidual cDNA library. The authenticity of the cDNA was verified by in vitro transcription/translation experiments and by the identity of the 10 N-terminal amino acids deduced for the mature peptide with those obtained by direct protein sequencing. The amino acid sequence indicates that pre-IGF-BP/PP12 consists of 259 amino acid residues. The putative signal peptide is 25 residues long, and the mature protein thus contains 234 amino acids and has a molecular mass of 25 293 Da. The sequence is very cysteine-rich at the N-terminus after which there are regions of clustered Pro, Glu, Ser and Thr residues (so-called PEST regions), which exist in proteins with short half-lives. The amino acid sequence also includes an Arg-Gly-Asp tripeptide that may function as a cell recognition signal. The IGF-BP/PP12 gene encodes a single 1.6 kb mRNA species that is expressed in decidua, secretory endometrium, liver and a human hepatoma cell line (HepG2). Southern blot analysis suggests that there is a single IGF-BP/PP12 gene in the human genome.

【 授权许可】

Unknown