Cross‐linking of collagen CNBr peptides by ozone or UV light

FEBS Letters

Fujimori, Eiji¹ 

[1] Department of Fine Structure Research, Boston Biomedical Research Institute, 20 Staniford St, Boston, MA 02114, USA

关键词: Collagen;    Crosslinking;    Peptide;    Fluorescence;    Ozone;    Ultraviolet irradiation;   

DOI  :  10.1016/0014-5793(88)81241-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Insoluble collagen from rat tail tendon was digested with cyanogen bromide. The resultant peptides were dissolved in 0.1% SDS solution and separated by gel filtration and gel electrophoresis. Cross-linking occurred in CNBr-cleaved peptides when they were exposed to ozone or biologically effective UV (300 nm) radiation. The enhancement of a blue fluorescence at 430 nm (excited at 350 nm) was found to be associated with oxidized, cross-linked peptides. Polymeric peptides, formed in collagen with aging, also exhibited enhanced blue fluorescence.

【 授权许可】

Unknown