The Kluyveromyces lactis KEX1 gene encodes a subtilisin‐type serine proteinase

FEBS Letters

Fukuhara, Hiroshi¹  Tanguy-Rougeau, Christine¹  Wésolowski-Louvel, Micheline¹ 

[1] Institut Curie, Section de Biologie, Bâtiment 110, Centre Universitaire, 91405 Orsay, France

关键词: Killer toxin;    Protein processing;    Nucleotide sequence;   

DOI  :  10.1016/0014-5793(88)80139-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

KEX1 is a chromosomal gene required for the production of the killer toxin encoded by the linear DNA plasmid pGKL-1 of Kluyveromyces lactis. The nucleotide sequence of the cloned KEX1 gene has been determined. The deduced structure of the KEX1 protein, 700 amino acids long, indicated that it contained an internal domain with a striking homology to the sequences of the subtilisin-type proteinases, and a probable transmembrane domain near the carboxyl terminus. The results confirm the hypothesis that the product of the gene KEX1 of K. lactis is a proteinase involved in the processing of the toxin precursor.

【 授权许可】

Unknown