Redox transformations of quinone antitumor drugs in liver microsomes

FEBS Letters

Rumyantseva, G.V.¹  Weiner, L.M.¹ 

[1] Institute of Chemical Kinetics and Combustion, 630090 Novosibirsk, USSR

关键词: Anthracycline antibiotic;    Spin trapping;    Hydroxyl radical;    ESR;   

DOI  :  10.1016/0014-5793(88)80138-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The hydroxyl radical has been spin trapped in microsomal and purified NADPH-cytochrome P-450 reductase systems in the presence of adriamycin, daunomycin and mitomycin C. The presence of a lag period in quinone-stimulated spin-adduct formation is associated with oxygen removal upon its reduction to H₂O₂. The hydroxy radical generation has been stimulated by the Fe-EDTA complex and completely inhibited by catalase. The mechanism of redox transformations of anthracyclines in a microsomal system has been proposed The single electron reduced quinone-containing anticancer antibiotics play the following roles: (i) they reduce oxygen to H₂O₂ and (ii) they reduce the ferric ions necessary for H₂O₂ decomposition with hydroxyl radical formation.

【 授权许可】

Unknown