Diacylglycerol stimulates phospholipase A2 from Swiss 3T3 fibroblasts

FEBS Letters

Burch, Ronald M.¹ 

[1] Nova Pharmaceutical Corporation, 6200 Freeport Centre, Baltimore, MD 21224, USA

关键词: Phospholipase A2;    Arachidonic acid;    Diacylglycerol;    Protein kinase C;    (Fibroblast);   

DOI  :  10.1016/0014-5793(88)80099-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We recently demonstrated that diacylglycerol induced arachidonate release and prostaglandin E₂ synthesis in 3T3 fibroblasts, and greatly augmented prostaglandin E₂ synthesis in response to submaximal and maximal concentrations of bradykinin. We have now partially purified a phospholipase A₂ from the cells. When phosphatidyl[³H]choline was used as substrate, several diacylglycerols augmented phospholipase A₂ activity. Diacylglycerol was effective at concentrations as low as 30 nM. Protein kinase C inhibition did not affect diacylglycerol's stimulation of phospholipase A₂. Diacylglycerol did not alter the calcium requirement for phospholipase A₂ or its pH optimum. The present study demonstrates that the effect of diacylglycerol to augment arachidonate metabolism is at the level of phospholipase A₂, itself.

【 授权许可】

Unknown