Solubilization of immune precipitates by complement in the absence of properdin or factor D

FEBS Letters

Späth, P.J.³  Pascual, M.²  Meyer-Hänni, Liselotte³  Schaad, U.B.¹  Schifferli, J.A.² 

[1] Children's Hospital, University of Bern, Bern, Switzerland;Clinique Médicale, Department of Medicine, Hôpital Cantonal Universitaire Geneva, Geneva Switzerland;Central Laboratory, Blood Transfusion Service, Swiss Red Cross, Bern Switzerland

关键词: Solubilization;    Immune complex;    Complement;    Properdin;    Factor D;   

DOI  :  10.1016/0014-5793(88)81318-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Various experiments have demonstrated that immune precipitates (IPs) are not solubilized by complement in the absence of alternative pathway function. To determine whether the characteristics of the IPs were responsible for these observations, we studied the solubilization (Sol) of IPs formed by bovine serum albumin (BSA)-rabbit antiBSA and tetanus toxoid (TT)-human antiTT. Sera deficient in properdin solubilized a fraction of BSA-antiBSA precipitates, although only when the IPs were formed in antibody excess. The same sera solubilized TT-antiTT precipitates with some delay but almost as efficiently as normal serum. Factor D-depleted serum solubilized a fraction of TT-antiTT precipitates too, indicating that Sol may proceed through activation of the classical pathway only. Thus, the requirements for complement-mediated Sol depend on the characteristics of the IPs and do not necessarily include alternative pathway function.

【 授权许可】

Unknown