Insulin‐like growth factor 1 and insulin reduce epidermal growth factor binding to Swiss 3T3 cells by an indirect mechanism that is apparently independent of protein kinase C

FEBS Letters

Brown, Kenneth D.¹  Corps, Anthony N.¹ 

[1] Department of Biochemistry, AFRC Institute of Animal Physiology & Genetics Research, Babraham, Cambridge CB2 4AT, England

关键词: Insulin-like growth factor 1;    Insulin;    Protein kinase C;    Epidermal growth factor receptor;    EGF;    epidermal growth factor;    IGF;    insulin-like growth factor;    IP3;    inositol 1;    4;    5-trisphosphate;    PBS;    phosphate-buffered saline;    PDBu;    phorbol 12;    13-dibutyrate;    PIP2;    phosphatidylinositol 4;    5-bisphosphate;    TPA;    12-O-tetradecanoyl phorbol 13-acetate;   

DOI  :  10.1016/0014-5793(88)80447-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Insulin-like growth factor 1 and insulin reduced the binding of ¹²⁵I-labelled epidermal growth factor (¹²⁵I-EGF) to Swiss 3T3 cells by 15–20% at 37°C, but not at 4°C. Scatchard analysis indicated that IGF-1 and insulin affected the higher-affinity component of EGF binding, an effect previously associated with the activation of protein kinase C. However, the inhibition of ¹²⁵I-EGF binding by IGF-1 and insulin was increased, not reduced, when the cells were treated with high concentrations of phorbol esters to down-modulate protein kinase C. We suggest that IGF-1 and insulin activate a protein kinase with similar or overlapping specificity to that of protein kinase C.

【 授权许可】

Unknown