Specific binding of [3H]retinoids to cellular retinoid‐binding proteins

FEBS Letters

Fujiki, Hirota²  Suganuma, Masami²  Ninomiya, Mitsuo²  Paik, Nam Sun²  Muto, Yasutoshi¹ 

[1] First Department of Internal Medicine, Gifu University School of Medicine, Gifu 500, Japan;National Cancer Center Research Institute, Tokyo 104, Japan

关键词: Retinol-binding protein;    Retinoic acid-binding protein;    Retinoid binding;    CRBP;    cellular retinol-binding protein;    CRABP;    cellular retinoic acid-binding protein;    HPLC;    high-performance liquid chromatography;    SDS-PAGE;    SDS-poly-acrylamide gel electrophoresis;   

DOI  :  10.1016/0014-5793(88)80437-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The specific binding of [³H]retinoids to cellular retinoid-binding proteins was measured directly by the cold acetone filtration method. After incubation of purified cellular retinoid-binding proteins with [³H]retinoids with or without competitors for 2–4 h, bound ligands were separated from free by filtration using cold acetone. Nonspecific binding of the ligands was reduced sufficiently to allow measurement of specific binding of [³H]retinoids to cellular retinoid-binding proteins. This method has the advantages of being rapid and practical and giving reproducible results.

【 授权许可】

Unknown