Regulation of the β2‐adrenergic receptor and its mRNA in the rat ventral prostate by testosterone

FEBS Letters

Lefkowitz, Robert J.²  Quarmby, Valerie E.¹  Collins, Sheila²  French, Frank S.¹  Caron, Marc G.² 

[1] The Laboratories for Reproductive Biology, The University of North Carolina, Chapel Hill, NC 27599, USA;Departments of Medicine (Cardiology), Biochemistry and Physiology, Howard Hughes Medical Institute, Duke University Medical Center, Durham, NC 27710, USA

关键词: β2-Adrenergic receptor;    Androgen;    Ventral prostate;    mRNA;    βAR;    β-adrenergic receptor;    β2AR;    β2-adrenergic receptor subtype;    CYP;    cyanopindolol;    SSC;    0.15 M NaCl;    0.015 M sodium citrate;    pH 7.0;   

DOI  :  10.1016/0014-5793(88)81378-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

To investigate the regulation of β₂AR expression in rat ventral prostate the effects of castration and testosterone replacement on the β₂AR were studied by ligand binding and Northern blot analysis. Orchidectomy depressed β₂AR number by 50% within 4 days and testosterone administration to 4-day castrates produced a rapid and complete recovery of β₂AR number within 24 h. In contrast to receptor number, β₂AR mRNA levels did not change relative to total RNA following castration. However, during the testosterone replacement period β₂AR mRNA levels rose transiently, reaching a maximum (3.5-fold) between 8 and 12 h, and this increase in mRNA preceded the recovery in β₂AR number in the membrane. Regulation of β₂AR gene expression by testosterone in the ventral prostate is thus complex and probably involves both transcriptional and post-transcriptional components.

【 授权许可】

Unknown