FEBS Letters | |
Subunit‐specific phosphorylation of pyruvate kinase in medullary thyroid carcinomas of the rat | |
Oskam, Ralph1  van der Heijden, Margreet C.M.1  Staal, Gerard E.J.1  Rijksen, Gert1  | |
[1] University Hospital Utrecht, Department of Hematology, Laboratory of Medical Enzymology, PO Box 16250, 3500 CG Utrecht, The Netherlands | |
关键词: Pyruvate kinase; Isoenzyme; Purification; Phosphorylation; (Rat medullary thyroid carcinoma; Tumor); AMTC; anaplastic (undifferentiated) medullary thyroid carcinoma; DMTC; differentiated medullary thyroid carcinoma; FDP; fructose-1; 6-bisphosphate; | |
DOI : 10.1016/0014-5793(88)81357-7 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Pyruvate kinase from anaplastic medullary thyroid carcinomas contains predominantly K-type subunits, whereas pyruvate kinase from differentiated medullary thyroid carcinomas consist of M- and K-type subunits in about equal proportion. In order to analyse the incorporation of phosphate in the respective isozymes after endogenous phosphorylation of cytosolic extracts with [32P]ATP, homotetrameric isozymes as well as heterotetrameric hybrids of differentiated0 tumors were resolved by affinity chromatography on Blue-Sepharose CL-6B and, if necessary, further purified by immunoprecipitation. SDS-polyacrylamide gel electrophoresis of purified isozymes and subsequent autoradiography showed the incorporation of phosphate in the K4-type isozymes, but not in the other isozymes. The phosphorylation appeared to be cAMP-independent and occurred on a serine residue.
【 授权许可】
Unknown
【 预 览 】
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RO201912020290628ZK.pdf | 471KB | download |