Posttranscriptional regulation of the expression of CAD gene during differentiation of F9 teratocarcinoma cells by induction with retinoic acid and dibutyryl cyclic AMP

FEBS Letters

Davidson, Jeffrey N.²  Church, Robert L.¹  Rao, Gadiparthi N.¹ 

[1] Department of Ophthalmology, Emory University School of Medicine, 1327 Clifton Rd NE, Atlanta, GA 30322 USA;Department of Microbiology and Immunology, University of Kentucky Medical Center, Lexington, KY 40536, USA

关键词: CAD gene;    mRNA;    Enzyme activity;    Aspartate transcarbamylase;    Cell growth;    Differentiation;    Pyrimidine;   

DOI  :  10.1016/0014-5793(88)80424-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have studied the regulation of expression of the carbamoyl-phosphate synthetase II-aspartate transcarbamylase-dihydroorotase gene in F9 teratocarcinoma cells during their differentiation into parietal endoderm cells by induction with a combination of retinoic acid and dibutyryl cyclic AMP. Steady-state levels of CAD mRNA decreased by 7-fold in F9 cells following 120 h of retinoic acid and dibutyryl cyclic AMP induction as compared to levels in uninduced cells. Conversely, no apparent changes were found in the steady-state levels of β-actin mRNA between induced and uninduced cells. Despite a 7-fold decrease in the steady-state levels of CAD mRNA, its rate of transcription remained the same between induced and uninduced cells, indicating a role for posttranscriptional mechanisms for its down regulation during retinoic acid- and dibutyryl cyclic AMP-induced differentiation of F9 cells. The cellular growth rate of F9 cells as determined by [³H]thymidine uptake and parallel cell counting decreased markedly during their induction with retinoic acid and dibutyryl cyclic AMP. Taken together, it is apparent that the expression of the CAD gene is cell-growth-dependent and its regulation in this system is at the posttranscriptional level.

【 授权许可】

Unknown