Amplification of the rudimentary gene in a PALA‐resistant Drosophila cell line

FEBS Letters

Laval, Monique¹  Giorgi, Dominique¹  Pardo, Daniel¹ 

[1] CNRS-LGBC, case 907, 13288 Marseille Cédex 09, France

关键词: Aspartate transcarbamylase;    N-PALA resistance;    Drosophila melanogaster;    Rudimentary gene;    Enzyme overproduction;    Gene amplification;   

DOI  :  10.1016/0014-5793(83)80790-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In Drosophila melanogaster the rudimentary locus encodes for a multifunctional protein catalyzing the first three enzymatic activities of pyrimidine biosynthesis. Cell lines were selected which were resistant to PALA (N-(phosphonoacetyl)-L-aspartate), a specific inhibitor of aspartate transcarbamylase, the second enzyme of this pathway. In a cell line where the enzyme production is increased 5 times, Southern blot analyses show that the rudimentary gene and surrounding regions are amplified about 5 times. In this case gene amplification could therefore account for the observed enzyme overproduction.

【 授权许可】

Unknown