A monoclonal anti‐thromboxane B2 antibody

FEBS Letters

Brune, Kay¹  Reinke, Margot¹ 

[1] Department of Pharmacology, University of Erlangen-Nürnberg, Universitätsstrasse 22, D-8520 Erlangen, FRG

关键词: Thromboxane B2;    Monoclonal antibody;    Radioimmunoassay;   

DOI  :  10.1016/0014-5793(88)80383-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A monoclonal antibody against thromboxane B₂ which may be used in standard fluid phase radioimmunoassays with a detection limit of around 40 pg and a binding affinity of 1.98 × 10⁹ M⁻¹ is described. Limited crossreactivity could be observed only with structurally closely related compounds such as 2,3-dinor-thromboxane B₂ (8.9%), thromboxane B₁ (15.7%) and thromboxane B₃ (39.7%). Detectable crossreactivity with 11-dehydro-thromboxane B₂ ω-carboxy-thromboxane B₂-hydroxy-thromboxane B₂, prostaglandins of the D-, E- and F-type as well as metabolites of prostacyclin was lacking. The monoclonal anti-thromboxane B₂ antibody proved well suited for measuring the thromboxane B₂ content in tissue culture supernatants as well as in human serum.

【 授权许可】

Unknown