Post‐translational modification of the β‐subunit of the human fibronectin receptor

FEBS Letters

Spaepen, Marijke¹  van Leuven, Fred¹  van den Berghe, Herman¹  Cassiman, Jean-Jacques¹  David, Guido¹  Jaspers, Martine¹  de Strooper, Bart¹ 

[1] Center for Human Genetics, University of Leuven, Campus Gasthuisberg, O&N6, Herestraat, B-3000 Leuven, Belgium

关键词: Post-translational modification;    Fibronectin receptor;    (Human);    Mab;    monoclonal antibody;    SA;    specific activity;    DME;    Dulbecco's modified Eagle's medium;    PBS;    phosphate-buffered saline;    SDS-PAGE;    SDS-polyacrylamide gel electrophoresis;    Endo H;    endoglycosidase H;    PA-bead;    protein A-Sepharose beads;    RaM;    rabbit anti-mouse IgG;    NHF;    normal human skin fibroblasts;   

DOI  :  10.1016/0014-5793(88)80859-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Monoclonal antibody DH12, directed against the β-subunit of the fibronectin receptor recognizes a doublet of proteins (100 and 110 kDa) in Western blots of solubilized whole fibroblasts. Pulse-chase experiments with [³⁵S]methionine in human skin fibroblasts suggested that the two proteins might be metabolically related as precursor (100 kDa) and product (110 kDa). Endo H digestion and [³H]fucose labeling suggested that maturation converted the high-mannose oligosaccharides (100 kDa) to the endoglycosidase H resistant complex type (110 kDa). This was supported by N-glycanase digestion and by chemical deglycosylation which showed a single polypeptide. Surface iodination of intact cells labeled only the presumed mature β-subunit.

【 授权许可】

Unknown