Bovine seminal plasmin is a DNA unwinding protein

FEBS Letters

Chatterji, Dipankar¹  Kumar, K.Prasanna¹  Gopal, Vijaya¹ 

[1]Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 0071 India

关键词: Topoisomer;    Unwinding;    Seminal plasmin;    RNA polymerase;    Elongation;   

DOI  :  10.1016/0014-5793(88)80856-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The duplex DNA unwinding ability of seminal plasmin from bovine semen was examined by treatment of plasmid-protein complexes with calf thymus topoisomerase I and resolution of the topoisomer distributions by agarose gel electrophoresis. Binding of seminal plasmin results in a moderate degree of unwinding of supercoiled plasmid. The elongation of the RNA chain by E.coli RNA polymerase over promoter containing template is not inhibited by seminal plasmin. However, the reinitiation of transcription is blocked in such cases indicating that seminal plasmin inhibits transcription by binding to the initiation site of RNA polymerase.

【 授权许可】

Unknown