Resolution and reconstitution of the rec BC deoxyribonuclease of Escherichia coli

FEBS Letters

Banfalvi, G.¹  Antoni, F.¹  Csuzi, S.¹ 

[1] Institute of Biochemistry, Dept. I. Semmelweis, University Medical School, PO Box 260, H-1444 Budapest, Hungary

关键词: Recombination;    Unwinding;    ATPase;    DNase;   

DOI  :  10.1016/0014-5793(83)80012-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The inactivation of rec BC nuclease activity and simultaneously the separation of 3 DNA-dependent ATPases and an ATP-independent DNases specific for single-stranded DNA have been observed after DEAE-cellulose chromatography of cell extracts from Escherichia coli. Two of the ATPases catalyze the strand separation of duplex DNA. Reconstitution of ATP-dependent DNase activity has been carried out by the combination of the separation enzymes.

【 授权许可】

Unknown