A zebrafish engrailed‐like homeobox sequence expressed during embryogenesis

FEBS Letters

Eiken, Hans G.¹  Hordvik, Ivar¹  Fjose, Anders¹  Molven, Anders¹  Njølstad, Pål R.¹ 

[1] Laboratory for Biotechnology, University of Bergen, PO Box 3152, Årstad, N-5029 Bergen, Norway

关键词: Engrailed gene;    Homeobox;    Protein homology;    Embryogenesis;    (Brachydanio rerio);   

DOI  :  10.1016/0014-5793(88)80849-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The zebrafish genome was found to contain two sequences which cross-hybridize strongly with the engrailed gene of Drosophila. Several independent clones containing one of these cross-hybridizing sequences were isolated from a zebrafish genomic library. Characterization of this region (ZF-EN) by DNA sequencing showed that it shares about 70% sequence identity with the engrailed homeobox. More extensive homeobox homology (90%) was found relative to the murine En genes. The closest relationship exists between ZF-EN and En-2 where the C-terminal domains (104 amino acids) encoded by these genes are almost identical. We also observed that ZF-EN and En-2 are very similar with respect to their transcript sizes and temporal expression patterns.

【 授权许可】

Unknown