NADPH‐dependent metabolism of the ribulose bisphosphate carboxylase/oxygenase inhibitor 2‐carboxyarabinitol 1‐phosphate by a chloroplast protein

FEBS Letters

Bowes, George²  Salvucci, Michael E.¹  Anderson, Joan C.¹  Holbrook, Gabriel P.² 

[1] United States Department of Agriculture, Agricultural Research Service, University of Kentucky, Lexington, KY 40546 USA;Department of Botany, University of Florida, Gainesville, FL 32611, USA

关键词: Carboxyarabinitol 1-phosphate;    Ribulose bisphosphate carboxylase;    NADPH;    Light regulation;    Photosynthesis;    (Tobacco);    CA 1-P;    2-carboxyarabinitol 1-phosphate;    Rubisco;    ribulose-1;    5-bisphosphate carboxylase/oxygenase;    RuBP;    ribulose 1;    5-bisphosphate;   

DOI  :  10.1016/0014-5793(88)80730-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Metabolism of 2-carboxyarabinitol 1-phosphate (CA 1-P), an endogenous inhibitor of ribulose bisphosphate carboxylase/oxygenase, occurs in the light. A soluble protein fraction which metabolized CA 1-P in the presence of NADPH was isolated from tobacco chloroplasts. A similar fraction from spinach exhibited much lower activity. The activity in tobacco extracts was stable overnight at 4°C but its maintenance during storage required dithiothreitol. The tobacco protein responsible for CA 1-P metabolism was partially purified by ion-exchange FPLC of stromal extracts. The requirements for NADPH and dithiothreitol for activity of this protein suggest a mechanism for the light-dependent control of CA 1-P levels in plants.

【 授权许可】

Unknown