Processing by inverted plasma membrane vesicles of in vitro synthesized major lipoprotein from Escherichia coli

FEBS Letters

Müller, Matthias¹  Krishnabhakdi, Siegfried S.¹ 

[1] The Institute of Biochemistry, University of Freiburg, Hermann-Herder-Str. 7, D-7800 Freiburg, FRG

关键词: Protein processing;    Lipoprotein;    Inside-out plasma membrane vesicle;    Protein synthesis;    (Escherichia coli);    SPase;    signal peptidase;    INV;    inverted plasma membrane vesicle;    SDS-urea-PAGE;    polyacrylamide gel electrophoresis in the presence of SDS and urea;   

DOI  :  10.1016/0014-5793(88)80710-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Synthesis, lipid modification and proteolytic processing of the major lipoprotein from Escherichia coli is shown to occur in a homologous in vitro transcription-translation system containing inverted plasma membrane vesicles. The primary translation product (cross-reacting with anti-lipoprotein antiserum) is a precursor which is converted into a lower molecular mass species of the size of mature lipoprotein by the addition of inverted membrane vesicles from E. coli. Conversion is prevented by globomycin, a specific inhibitor of the unique lipoprotein-signal peptidase II, which is active only on lipid-containing precursors. The inverted plasma membrane vesicles used here must therefore contain active lipid-modifying enzymes and signal peptidase II.

【 授权许可】

Unknown