Occurrence and possible roles of acetoacetyl‐CoA thiolase and 3‐ketoacyl‐CoA thiolase in peroxisomes of an n‐alkane‐grown yeast, Candida tropicalis

FEBS Letters

Kurihara, Tatsuo¹  Tanaka, Atsuo¹  Ueda, Mitsuyoshi¹ 

[1] Department of Industrial Chemistry, Faculty of Engineering, Kyoto University, Yoshida, Sakyo-ku, Kyoto 606, Japan

关键词: Acetoacetyl-CoA thiolase;    3-Ketoacyl-CoA thiolase;    β-Oxidation system;    Peroxisome;    (Alkane-grown yeast;    Candida tropicalis);   

DOI  :  10.1016/0014-5793(88)80830-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Two kinds of 3-ketoacyl-CoA thiolases were found in the peroxisomes of Candida tropicalis cells grown on n-alkanes (C₁₀◀C₁₃). One was a typical acetoacetyl-CoA thiolase specific only to acetoacetyl-CoA, while another was 3-ketoacyl-CoA thiolase showing high activities on the longer chain substrates. A high level of the latter thiolase activity in alkane-grown cells was similar to that of other enzymes constituting the fatty acid β-oxidation system in yeast peroxisomes. These facts suggest that the complete degradation of fatty acids to acetyl-CoA is carried out in yeast peroxisomes by the cooperative contribution of acetoacetyl-CoA thiolase and 3-ketoacyl-CoA thiolase.

【 授权许可】

Unknown