Psoralen photofootprinting of protein‐binding sites on DNA

FEBS Letters

Jeppesen, Claus¹  Zhen, Wei-ping¹  Nielsen, Peter E.¹ 

[1] Department of Biochemistry B, The Panum Institute, University of Copenhagen, Blegdamsvej 3, DK 2200 N, Copenhagen, Denmark

关键词: Psoralen;    Footprint;    RNA polymerase;    Promoter;    λ-repressor;    Photocrosslinking;   

DOI  :  10.1016/0014-5793(88)80800-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Using a BAL31 exonuclease assay to determine the sites of 4,5′,8-trimethylpsoralen photocrosslinking in DNA we have shown that 5′-TA sites which are accessible to psoralen DNA interstrand photocrosslinking in naked DNA become inaccessible when protein, in casu, λ-repressor E. coli or RNA polymerase are bound at their recognition DNA sequences (O_R1 operator or deol promoter, respectively). These results show that psoralens can be used as photofootprinting reagents to study specific protein-DNA interactions.

【 授权许可】

Unknown