Limited proteolysis of actin by a specific bacterial protease

FEBS Letters

Smirnova, T.D.¹  Usmanova, A.M.¹  Khaitlina, S.Yu.¹ 

[1] Institute of Cytology, USSR Academy of Sciences, Leningrad 194064, USSR

关键词: Actin;    Actin proteolysis;    DNase I;    Proteinase;   

DOI  :  10.1016/0014-5793(88)80610-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A 36 kDa fragment of rabbit skeletal muscle actin resistant to further proteolytic breakdown was obtained with a new bacterial protease. This fragment was the only cleavage product obtained from native actin whereas proteolysis of heat-inactivated actin was unlimited. The 36 kDa fragment failed to polymerize and to inhibit DNase I activity. Binding to DNase I protects actin against proteolysis by protease. The results on actin proteolysis by different proteases are compared.

【 授权许可】

Unknown