Evidence for the presence of DNase—actin complex in L1210 leukemia cells

FEBS Letters

Malicka-Blaszkiewicz, Maria¹  Roth, Jay S.¹ 

[1] Section of Biochemistry and Biophysics, Biological Sciences Group, University of Connecticut, Storrs, CT 06268, USA

关键词: DNase I;    Actin;    DNase—actin complex;    DNase inhibition;    Latent DNase;    Leukemia L1210;    SDS;    sodium dodecyl sulfate;    pCMB;    p-chloromercuricbenzoic acid;   

DOI  :  10.1016/0014-5793(83)80155-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

L1210 leukemia cell cytosol was analysed for the presence of DNase I activity. No free activity was determined in crude cytosol. DNase I enzyme was found to occur in a latent form bound to cytoplasmic actin. DNase-actin complex was partially isolated by Sephadex filtration and DNase I-like activity was demonstrated after SDS gel electrophoresis of the complex and enzyme renaturation. The results were compared with those for synthetic complex of pancreatic bovine DNase I and chicken muscle actin.

【 授权许可】

Unknown